Ready-to-Assay EP2 Frozen Cells

Code: HTS185RTA D2-231

Application

Human EP2 GPCR frozen cells for Calcium Flux FLIPR Assays.

Biochem/physiol Actions

Target Sub-Family: Prostanoid

Protein Target...


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Your Price
€1,274.00 EACH
€1,567.02 inc. VAT

Application

Human EP2 GPCR frozen cells for Calcium Flux FLIPR Assays.

Biochem/physiol Actions

Target Sub-Family: Prostanoid

Protein Target: EP2

GPCR Class: A

Cell Line Description

GPCR Cell Lines

Host cells: Chem-9

Components

Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.Fifty (50) mL of Media Component.

General description

Millipore's Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

Prostanoids bind to a family of 8 GPCRs to exert their biological effects (Narumiya and FitzGerald, 2001). The protanoid PGE2 causes pain, vasodilation, immunosuppression of T cells, bone resorption and promotion of carcinogenesis. Four related GPCRs, EP1, EP2, EP3 and EP4, each bind to PGE2, but the different G protein coupling status of each receptor leads to distinct biological effects. EP2 couples primarily to Gs to increase intracellular cAMP levels. Mice deficient in EP2 receptor showed impaired ovulation and fertilization, salt-sensitive hypertension (Kennedy et al., 1999). It has been shown that EP2 receptors are also involved in cancer associated immunodeficiency. Thus, genetic knockout of the EP2 receptor reduced tumor growth and prolonged survival in mice that had undergone isograft injection of MC26 or Lewis lung carcinoma cells (Yang et al., 2003). Millipore's cloned human EP2-expressing cell line is made in the Chem-9 host, which supports high levels of recombinant EP2 expression on the cell surface and contains high levels of the promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists and antagonists at EP2.

biological sourcehuman
detection methodfluorometric
manufacturer/tradenameReady-to-Assay
NCBI accession no.NM_000956
Quality Level100
shipped indry ice
technique(s)calcium flux assay: suitable
Hazard Class9
Un Number3245
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